Bahan Reagen

Nama ReagenSpesifikasiHarga (Rp.)Volume
1st antibody solution0,1-0,2% BSA5.100mL
2nd antibody solution0,1-0,2% BSA9.20010 mL
Acrylamide solution30%1.300mL
Ammonium Persulfate (APS)10%3.000mL
Blocking solution (BSA)0,1-1%64.10010 mL
Blocking solution (Skim milk)0,1-1%6.10010 mL
Bromophenol1%1.800mL
DTT1 mM3.500mL
Glycerol50%3.700mL
Lysis BufferRIPA-cell73.500mL
Lysis BufferSDS-Tissue62.800mL
PBST0,10%500mL
Ponceau S4.1001x
Resolving Buffer1.500mL
Running Buffer10X330.000500 mL
1X23.9001x
71.700500 mL
Sample buffer5.400mL
SDS10%2.400mL
SDS Gel5-7,5%42.6001 gel
SDS Gel10%55.8001 gel
SDS Gel15%70.2001 gel
Sodium hydroxide (NaOH)10%200mL
Transfer buffer10X227.000500 mL
1X82.6001X
165.200500 mL
Tris-Acetate-EDTA (TAE)1x1.10010 mL
Tris-EDTA (TE) Buffer1x1.00010 mL
Tris-HCl1 M pH 6,81.300mL
Tris-HCl1,5 M pH 8,81.700mL